Experiments carried out through 2016 revealed that a proper protocol for the in vitro propagation of Koronaki and Coratina olive cvs. as follows : Sterilization of stem node explants was carried out successfuly with copper sulphate at 50mg/l for Koronaki cv. And Ampicillin at 150mg/l for Coratina cv. both cultured on Olive medium.Multiplication on MS medium enriched with BAP at 1.5mg/l for both cvs.  Rooting of Koronaki on half sterngth OM medium enriched with IBA at 0.5mg/l and Coratina on half sterngth MS medium enriched with NAA at 0.1mg/l.  Acclimization of Koronaki plantlets derived from half strength OM rooting medium and acclimatized on peat : sand  (1:1) and Coratina plantlets derived from MS rooting medium and acclimatized on peat :sand : vermiculite (1:1:1).