Proline's content was estimated in different organs of durum wheat; dry wheat seeds (caryopsis), seeds during germination, second and third leave's level in different degrees of water alimentation: 75%, 50.0, 42.5, 35.0, 25.0 and 12.5% of the field capacity. Four genotypes of durum wheat Triticum durum DESF were studied from three different origins; Algeria, France and Mexico. Results showed that proline's content was low in dry wheat and in seeds during germination, and increased by decrease of water supplies. This amino acid estimation during experimentation, allowed us to classify the studied genotypes into 3 main groups: - Genotypes with high proline accumulation (Mexicali). - Genotypes with low proline accumulation (Clairdoc). - Two intermediate genotypes MBB and OZ. These differences among genotypes can be exploited in parietal selection with relation towater deficit.