Twenty nine actinomycetes isolates were isolated from Burullos Lake and characterized taxonomically for 62 phenotypic traits including morphological; biochemical, nutritional, substrate utilization and anti-microbial activities.  The results were analyzed by numerical techniques using the simple matching coefficient (S_SM) and UPGMA clustering. At 54% similarity level, the majority of the isolates were grouped into six phena (A, B, C, D, E and F). Only two isolates were grouped separately and formed two single clusters at the same level of similarity. A representative isolate from each phenon was identified. The isolates were found to be Streptoverticillum morookaense, Nocardia brasiliensis, Streptomyces alanosinicus, Streptomyces globosus and Streptomyces gancidicus