Two field experiments were carried out during the two successive seasons of 2005 and 2006 at Banha (Qalubia Governorate) to study the effect of three rates of poultry manures (50, 75 and 100 N unit/fed.) and the biofertilizer nitrogen (nitroben) on growth, yield, quality and chemical composi-tion of squash. Obtained data showed that using 100 N unit/fed. from poultry manure gave the highest vegetative growth characters, yield and quality. Application 100 N unit/fed. from poultry manure also increased the nitrogen percentage and heavy metals in squash tissues. In addition, using nitroben biofertilizer gave the highest vegetative growth characters, yield, quality and nitrogen per-centage. On the contrary, nitroben biofertilizer reduced the heavy metals in squash tissues