The response of embryogenic callus of date palm cvs. (Pertomuda and Skooty) was tested on media containing two types of silver (STS and Ag-NO3) alone or combined with 0.25 & 0.5 mg/l 2ip. Addition of STS onto callus induction medium sig-nificantly enhanced embryogenic callus production (both growth and induction). AgNO3 as a heavy metal ion made a stress treatment on callus to proliferation embryos than STS, this proliferation was increased at a low concentration of AgNO3, but in the presence of 2ip gave the opposite trend, the embryos increased at 4 mg/l or 6 mg/l AgNO3 + 0.5 mg/l 2ip and proceeded normally embryos for both Pertomuda and Skooty date palm.
Abbreviations: STS = Silver thiosulphate; EC = embryogenic callus; NAA = α-naphthaleneacetic acid and 2ip = N6-2-isopentenyladenine.