The peanut (Arachis hypogaea L.) is an important oilseed crop in tropical and subtropical regions of the world.Oil content has an important quality trait for peanut. However, the progress in genetic improvement of oil content is slow. Therefore, identification of molecular markers for oil content trait is  a great impact in molecular breeding. Nineteen RAPD and  ten SSR primers  were used to detect markers related to  oil content in peanut. The five peanut cultivars were grown for two seasons (2013, 2014) in   El-Nubaria, Al-Beheira Governorate, and Egypt. The results showed that, Gregory cultivar recorded the highest value of oil content, while Giza5 cultivar exhibited the lowest value of oil content in both seasons.  The results indicated the presence of five positive and nine negative RAPD markers and  two positive and  one negative SSR markers that could be considered as reliable markers for oil content  in peanut.