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256736

Application of Entomopathogenic Nematodes to Control Larvae of Temnorhynchus baal (Reiche and Saulcy) Under Laboratory Conditions and In Strawberry Fields

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Last updated: 22 Jan 2023

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Abstract

The white grub, Temnorhynchus baal, is a major pest of the strawberry crop in many Asian and African countries. The white grabs cause significant economic losses in the yield. The present study aims to evaluate the effectiveness of entomopathogenic nematodes (EPNs) on the third larval instar of T. baal under laboratory conditions. The study extended to compare the efficacy of a combination of EPNs (Steinernema glaseri+Heterorhabditis bacteriophora) against a natural infestation of T. baal with an organophosphorus insecticide (Diazinon) in an Egyptian strawberry field. In the laboratory, five larvae of T. baal were placed in soil and infected with EPNs. At the highest concentration of 4000 IJs/larva, the larvae were more susceptible to infection with S. glaseri than H. bacteriophora, with mortality percentages of 96 and 88%, respectively. The LC50 of S. glaseri and H. bacteriophora was 937.44 and 1026.58 IJs/larvae, respectively. The mortality percentage was 96% and 100% when treated for five larvae/cup and one larva/cup, respectively, after being infected with a combination of S. glaseri+ H. bacteriophora. The mortality percentage was higher in the mixture than for each species studied individually. In the field, throughout the seasons, the percentage of wilted plants was 17.64, 66.09, and 83.95% in 2020 and 12.56, 67.87, and 75.62% in 2021, for the plots treated with a combination of EPNs, insecticides, and control, respectively. Present findings indicate that entomopathogenic nematodes are good alternatives to control the white grub, T. baal, in strawberry fields.

DOI

10.21608/eajbsa.2022.256736

Keywords

white grub, Heterorhabditis bacteriophora, Steinernema glaseri, Organophosphorus insecticide

Authors

First Name

Dalia

Last Name

Adly

MiddleName

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Affiliation

Biological Control Department, Plant Protection Research Institute, Agricultural Research Center, Giza, Egypt.

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City

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Orcid

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First Name

Gehan

Last Name

Nouh

MiddleName

M.

Affiliation

Biological Control Department, Plant Protection Research Institute, Agricultural Research Center, Giza, Egypt.

Email

gehannoah@yahoo.com

City

-

Orcid

-

First Name

Aziza

Last Name

Eid

MiddleName

E.

Affiliation

Biological Control Department, Plant Protection Research Institute, Agricultural Research Center, Giza, Egypt

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Orcid

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Volume

15

Article Issue

3

Related Issue

35649

Issue Date

2022-09-01

Receive Date

2022-06-19

Publish Date

2022-08-24

Page Start

67

Page End

77

Print ISSN

1687-8809

Online ISSN

2090-0813

Link

https://eajbsa.journals.ekb.eg/article_256736.html

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https://eajbsa.journals.ekb.eg/service?article_code=256736

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8

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Original Article

Type Code

667

Publication Type

Journal

Publication Title

Egyptian Academic Journal of Biological Sciences. A, Entomology

Publication Link

https://eajbsa.journals.ekb.eg/

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Article

Created At

22 Jan 2023