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Effect of Different Host Plants on The Different Haemocyte Counts and Haemocyte Viability of Larvae of Spodoptera littoralis and Agrotis ipsilon

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Last updated: 03 Jan 2025

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Abstract

The present study aimed to investigate the effect of different six host plants namely, Cabbage, alfalfa, Jaw's mallow, lettuce, turnip, and castor leaves on the total haemocyte count (THC), percentage of haemocyte viability, and the percentage of each type of haemocyte (differential haemocyte count, DHC) in Spodoptera littoralis and Agrotis ipsilon fourth instar larvae. There are five types of haemocytes in A. ipsilon and S. littoralis larvae; Prohaemocyte, Plasmatocyte, Granulocyte, Spherocytes, and Oenocyte. Results recorded the highest value 13.3±0.68 of Prohaemocyte at lettuce and the lowest value 9.3 for Cabbage, alfalfa and turnip. On the other hand, Prohaemocyte recorded the highest value 12.6 ± 0.9 in the case of castor feeding and 8.7 ± 0.3 for turnip for S. littoralis. Plasmatocyte recorded 45.3 ± 2.9 cells/mm3for A. ipsilon feed on turnip and 47.3 ± 1.8 cells/mm3for S. littoralis feed on lettuce as the highest value while Granulocyte recorded 33 ± 4.1 and 35 ± 0.7 for A. ipsilon feed on   Jaw's mallow, respectively. Spherocytes recoded 15.3 ± 0.9 and 12.3 ± 0.9 for A. ipsilon and S. littoralis feed on alfalfa. Finally, Oenocyte recorded 7.7 ± 1.5 and 7.3 ± 1.4 for A. ipsilon and S. littoralis feed on castor.  Total haemocytic count recorded 29 ± 0.3 and 36 ± 0.75 cells/mm3 as the highest value when both A. ipsilon and S. littoralis feed on Alfalfa. Also, the haemocyte viability % recorded the highest value 91.5 ± 2.6 and 96.2 ± 0.8 % for A. ipsilon and S. littoralis feed on feed on Alfalfa. The result recorded increasing DHC level after feeding of the fourth larval instar of S. littoralis and A. ipsilon on different plant hosts such an increase in DHC gives an impression that blood cells may share in detoxifying. The most obvious activity of haemocyte is phagocytosis. In this context, the most active phagocytes are the Plasmatocyte, Plasmatocyte which was implicated in the encapsulation of necrotic tissues. 

DOI

10.21608/eajbsa.2020.118768

Keywords

Haemocyte count, haemocyte viability, Spodoptera littoralis and Agrotis ipsilon larvae

Authors

First Name

El-Khayat,

Last Name

F.

MiddleName

E.

Affiliation

Faculty of Agriculture, Moshtohor, Benha University

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Orcid

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First Name

Hassan

Last Name

Dahi

MiddleName

F.

Affiliation

Plant Protection Research Institute, Agricult. Research Center, Dokki, Giza, Egypt

Email

hassandahi@yahoo.com

City

-

Orcid

-

First Name

Tawfik,

Last Name

M.

MiddleName

M.

Affiliation

Department of Plant Protection, Faculty. Agriculture, Tripoli University, Libya

Email

-

City

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Orcid

-

First Name

El-Shewy,

Last Name

M.

MiddleName

A.

Affiliation

Faculty of Agriculture, Moshtohor, Benha University

Email

-

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Orcid

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Volume

13

Article Issue

4

Related Issue

17651

Issue Date

2020-12-01

Receive Date

2020-07-04

Publish Date

2020-12-01

Page Start

57

Page End

63

Print ISSN

1687-8809

Online ISSN

2090-0813

Article File

EAJBSA_Volume 13_Issue 4_Pages 57-63.pdf

PDF . 679.2kB

Link

https://eajbsa.journals.ekb.eg/article_118768.html

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https://eajbsa.journals.ekb.eg/service?article_code=118768

Order

5

Type

Original Article

Type Code

667

Publication Type

Journal

Publication Title

Egyptian Academic Journal of Biological Sciences. A, Entomology

Publication Link

https://eajbsa.journals.ekb.eg/

MainTitle

Effect of Different Host Plants on The Different Haemocyte Counts and Haemocyte Viability of Larvae of Spodoptera littoralis and Agrotis ipsilon

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Article

Created At

22 Jan 2023