The efficacy of six essential oils compared with formic acid and Apistan stripes, against Varroa mite were evaluated. The infestation levels of these substances were evaluated in honeybee colonies during spring season, 2019 under arid regions environmental conditions. It is obvious that garlic oil was superior of all treatments in falling number of varroa mites after treatments with significant difference giving 46.66±3.92 during the experimental period. Formic acid, clove oil, and apistan strips were the most effective of fallen varroa mites after garlic oil with insignificant differences among them represented 26.33±0.33, 21.33±2.40, and 20.66±1.20 fallen mites/colony, respectively. On contrary, neem oil was the least in mean number of fallen varroa mites equalized with control colonies giving (0.33fallen mites/colony). For the reduction percentage on both brood and workers, the outstanding treatments were garlic oil, formic acid, and apistan strips represented 87.33, 87.33, and 82.56 %, respectively. On the other hand, the inferior reduction was observed for neem oil with mean reduction percentage 4.75 % for both brood and worker bees. This study indicates that using garlic oil, formic acid, and apistan strips mixture can be added to IPM programs for controlling Varroa mite.