Mosquito-borne diseases are amongst the most serious threat to humans, especially in tropical countries. Mosquito control has never been easy and has always posed several difficulties like hazards of chemicals used, resistance raised in mosquito populations or cost-effectiveness. Current control methods mainly depend on the use of conventional chemicals, Physical methods, biological methods, and transgenic mosquitoes. Currently, none of these single strategies is fully successful. Unique eco-friendly approaches to achieve mosquito vector control are immediately required. The plant-mediated creation of nanoparticles is helpful over chemical and physical methods since it is cheap, single-step, and easy to use. Recently the number of plant-based compounds have been proposed for efficient synthesis of metal nanoparticles effective against mosquitoes. The present study was an attempt to study the mosquitocidal potential of the bionanomaterial of the bark extract of Eucalyptis grandis with silver. The larvicidal activity after 24 hours was found to be LC ₅₀ = 42 and 35 ppm for the two species of the mosquitoes studied herein.