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Enhancing the Efficacy of Certain Spray-Dried Baculovirus (AgipMNPV) against Cutworm, Agrotis ipsilon Hufnagel (Lepidoptera: Noctuidae)

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Last updated: 03 Jan 2025

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Abstract

The insecticidal efficacy of various baculovirus formulations against the cutworm Agrotis ipsilon was determined. The treatments consisted of molasses from suagrcane mixing with each of wheat germ, biochar, talc, chitosan, silica gel, pyrolysis bio-oilaqueous phase, pyrolysis bio-oilorganic phase, calcium carbonate, cornflour, calcium cassinate, aluminium potasium sulphate, Diatomaceous earth, dextrin, lignin PC 1307, soy screen, blankphor, skim milk powder, potassium cassinate, magnsium chloride, date molasses and pregelatinized starch. All applied in spray-dried at 2.2 x 109  occlusion body(OB) mL-1. The bioassay of each spray-dried formulation using the droplet feeding method by the blue solution containing 2% sugar and 0.1% blue dye. The resulting suspensions should all contain 0.377 x 106 OB mL-1, represent the LD70 for the unformulated virus. Exposure newly hatched larvae of   cutwormsand transfer to individual diet cups for each treatment and incubated at 28oC in dark incubator for 7 days. The obtained results revealed that satisfactory control of the pest of 100% larval mortality compared to untreated control was achieved with the formulations containing molasses mixing with talc, silica gel, calcium carbonate, cornflour, calcium cassinate, diatomaceous earch, soy screen, and dextrin. However, the formulations containing chitosan, pyrolysis bio-oilaqueous phase and pyrolysis bio-oilorganic phase gave the lowest mortalities as 12.2, 11.1 and 6.7%, respectively. These findings is an attempt to provides an interesting alternative developed biopesticide formulations made with natural ingredients that could improve the efficacy and persistence of virus-based biopesticides.

DOI

10.21608/eajbsa.2020.76401

Keywords

Spray-dried formulations, Baculovirus (AgipMNPV), Agrotis ipsilon

Authors

First Name

Atef

Last Name

Sayed

MiddleName

M. M.

Affiliation

Plant Protection Research Institute, Agricultural Research Center, Dokki, Egypt.

Email

atef.mahmoud1@gmail.com

City

-

Orcid

-

First Name

Robert

Last Name

Behle

MiddleName

W.

Affiliation

United States Department of Agriculture, Agricultural Research Service, National Center for Agricultural Research, Crop Bioprotection Research Unit, Peoria, Illinois, USA.

Email

-

City

-

Orcid

-

First Name

Ghada

Last Name

El-Masry

MiddleName

N.

Affiliation

Plant Protection Research Institute, Agricultural Research Center, Dokki, Egypt

Email

-

City

-

Orcid

-

Volume

13

Article Issue

1

Related Issue

10290

Issue Date

2020-03-01

Receive Date

2020-02-16

Publish Date

2020-03-01

Page Start

123

Page End

133

Print ISSN

1687-8809

Online ISSN

2090-0813

Link

https://eajbsa.journals.ekb.eg/article_76401.html

Detail API

https://eajbsa.journals.ekb.eg/service?article_code=76401

Order

12

Type

Original Article

Type Code

667

Publication Type

Journal

Publication Title

Egyptian Academic Journal of Biological Sciences. A, Entomology

Publication Link

https://eajbsa.journals.ekb.eg/

MainTitle

Enhancing the Efficacy of Certain Spray-Dried Baculovirus (AgipMNPV) against Cutworm, Agrotis ipsilon Hufnagel (Lepidoptera: Noctuidae)

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Type

Article

Created At

22 Jan 2023