Field experiments were carried out of about 11 kirats planted with (Giza 86) cotton variety during seasons 2018 and 2019 on 15^th  July and 1^st, 15^th August in a field located at Qaha Research Station, Plant Protection  Research Institute, Qalyoupia governorate. The selected area was split into 10 plots and the control plot. Entomopathogenic fungi and a commercial formulation of  Bacillus thuringiensis (Berliner)  were sprayed by using  Hand-held Hydraulic sprayer (Matabi) (56 L./fed.) and Economy Micron ULVA (15 L./Fed.) on cotton field highly infested with cotton Spiny bollworm  Earias insulana  larvae. All tested Bio-Insecticides revealed a significant negative influence on Earias insulana  larvae. The most effective on reduction percentage of bolls infestation is Metarhizium anisopliae, Beauveria bassiana either isolates or (W. P.) with Economy Micron ULVA (15 L./Fed.)  followed by Bacillus thuringiensis (W.P.) with Hand-held Hydraulic sprayer (Matabi) (56 L./Fed.). It could be recommended to use these Bio-Insecticides with ULV spraying equipment with not less than (15L/Fed.). A satisfactory coverage was obtained on cotton plants. The spectrum of droplets ranging between 130-166 mm (VMD). With a sufficient number ranging from  24-170 N/cm². The rate of performance of  Hand-held Hydraulic sprayer (Matabi) (56 L./Fed.) was 3.5 Fed./day. It was the best equipment, but the lowest rate of performance was  Economy Micron ULVA (15 L./Fed.) since it could spray only 3 Fed./day. The results indicated that there was no significant difference between the isolate form of fungi and wettable powder in reduction percentages of bolls infestation. Data showed that bio-insecticides may be recommended in integrated pest management because of their safety on animals, man  and environment   Also, Low Volume spraying reducing the time lost in the process filling the machines with the spray solution and saving the lost spray on the ground.