This study was carried out to study the effect of infested rose plants by Icerya aegyptiaca (Douglas)(Margarodidae : Homoptera).(in different stages of infestation) on the physiological and natural characteristics of rose oil at two locations (governorates), International Garden (Cairo Governorate) and El-Orman Garden, (Giza Governorate) under glasshouse conditions during successive seasons 2018.
             Data obtained showed that the most important components of rose oil such as (geraniol, citronellol, nerol, stearpoten, phenyl ethanol and bioflavonoids), acids such as Citric acid and Malic acid and vitamins such as vitamin A, B, C and vitamin D were changed its concentrations as result of infestation by I. aegyptiaca. And high infestation by I. aegyptiaca affected concentrations of these components more than medium and low infestation, respectively compared to control. In addition, data obtained showed that the most natural characteristics of rose oil such as volatility, light rotation and refraction value were changed as a result of infestation by I. aegyptiaca but other natural characteristics like freezing point did not change after infestation by I. aegyptiaca. And high infestation by I. aegyptiaca affected the natural characteristics of rose oil more than medium and low infestation, respectively compared to control.