This study was carried out to study effect of infested Carnation flowers (Dianthus caryophyllus L.) by Carnation thrips, Haplothrips cottei (Vuillet) (Thysanoptera: Thripidae) andTetranychus urticae Koch (Acari: Tetranychidae) on the vase life period of Carnation flowers under glasshouse conditions at two locations (governorates), International Garden (Cairo Governorate) and El-Orman Garden, (Giza Governorate) during successive seasons 2018. This is because the vase life period is very important parameter in cut flowers. And there are many factors affected on the vase life period. Therefore this study divided into two parts, first part studied effect of infested Carnation flowers by H. cottei andT. urticae on the vase life period of Carnation flowers after picking. Second part studied effect of infested Carnation flowers by the same pests on the internal components of these flowers which correlated with vase life period such as total sugar and total protein. Results obtained showed that the infestation by H. cottei reduced the vase life period of carnation flowers after picking more the infestation byT. urticae compared to control (which non infested by the same pests). Also results showed that the infestation by H. cottei reduced total suger and total protein at the infested Carnation flowers more than the infestation byT. urticae compared to control. Lastly, results obtained showed that the infestation by H. cottei andT. urticae changed the number and arrange of the protein banding patterns (amino acids) of infested Carnation flowers petals compared to control.