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56312

Molecular Barcoding of Green Bottle Fly, Lucilia sericata (Diptera: Calliphoridae ) Using COI Gene Sequences In Upper Egypt.

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Last updated: 03 Jan 2025

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Abstract

The mitochondrial cytochrome oxidase I (COI) gene has been proposed as standard  DNA barcoding marker for the identification of organisms. COI provides an ideal species identification marker in insects, the partial mitochondrial gene of (COI) for Egyptian specimens 6 area (L. sericata ) of genus Lucilia were amplified and sequenced successfully from Egypt in addition to data collected from the Genbank from several representative taxa and neighboring countries.The obtained sequences were analyzed and edited using Bio Edit program and aligned using Basic Local Alignment Search Tool software (BLAST). Construction of phylogenetic trees was made using the Maximum Parsimony (MP) and Maximum Likelihood (ML). SDS-PAGE traditionally is a technique employed by biochemists to detect and characterize proteins in a mixture based on their molecular size. Protein was analyzed at 14% gel concentration for wide range detection of high and low molecular weight (MW) proteins. If analytical conditions are optimized and followed by sophisticated techniques, to detect any changes in protein patterns could be happened in six Egyptian specimens of  Lucilia sericata according to different areas.

DOI

10.21608/eajbsa.2019.56312

Keywords

cytochrome oxidase I, Lucilia sericata, Phylogeny, divergence

Authors

First Name

mohamed

Last Name

Elshehaby

MiddleName

Ibrahim

Affiliation

Zoology Department, Faculty of Science, Al Azhar University,Assiut, Egypt

Email

mohamedelshehaby44@yahoo.com

City

Assiut

Orcid

-

First Name

Mahran Tony

Last Name

Mahran Tony

MiddleName

-

Affiliation

Zoology Department, Faculty of Science, Al Azhar University,Assiut, Egypt

Email

-

City

-

Orcid

-

First Name

Abd elbaset

Last Name

Abd elreheem

MiddleName

M. A

Affiliation

Zoology Department, Faculty of Science, Al Azhar University,Assiut, Egypt

Email

-

City

-

Orcid

-

Volume

12

Article Issue

5

Related Issue

7206

Issue Date

2019-10-01

Receive Date

2019-10-08

Publish Date

2019-10-01

Page Start

111

Page End

123

Print ISSN

1687-8809

Online ISSN

2090-0813

Article File

EAJBSA_Volume 12_Issue 5_Pages 111-123.pdf

PDF . 978.2kB

Link

https://eajbsa.journals.ekb.eg/article_56312.html

Detail API

https://eajbsa.journals.ekb.eg/service?article_code=56312

Order

9

Type

Original Article

Type Code

667

Publication Type

Journal

Publication Title

Egyptian Academic Journal of Biological Sciences. A, Entomology

Publication Link

https://eajbsa.journals.ekb.eg/

MainTitle

Molecular Barcoding of Green Bottle Fly, Lucilia sericata (Diptera: Calliphoridae ) Using COI Gene Sequences In Upper Egypt.

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Article

Created At

22 Jan 2023