The present study dealt with evaluating the effect of whole body extracted by ethyle acetate of L. sericata, C. albiceps and M. domestica as exhibited antibacterial activity against some species of bacteria. They were shown to inhibit the growth of Gram–positive bacteria Staphylococcus aureus and Staphylococcus epidermidis, Gram–negative bacteria, Escherichia coli and  Klebsiella pneumoniae and Fungi, Aspergillus fumigatus thus exhibited limited inhibitory effect towards Gram–positive bacteria Bacillus subtilis and Gram–negative Pseudomonas aeruginosa and Fungi Syncephalastrum racemosum and Candida albicans, this effect was slowed down when challenged with secretion on a solid media but no zone of complete inhibition was detected. Growth inhibiting activity was determined in liquid growth media using the Gram–positive, Gram–negative bacterial and fungal strains as indicator organisms. The antiviral activity of whole body extracted by ethyle acetate of L. sericata, C. albiceps and M. domestica nES were tested against Vero cell line was developed for isolation and propagation of many enteroviruses and hepatitis A and average values were considered.