Green synthesized
nanoparticles have been studied as  new
and more effective biopesticides against insects of medical importance and this
for their eco-friendly aspects. In the current study Moringa oleifera
leaf extract (Mo-LE) was used to synthesis silver nanoparticles (Mo-AgNPs) and
zinc oxide nanoparticles (Mo-ZnONPs). Low concentrations of Mo-LE, Mo-AgNPs andMo-ZnONPs showed the larvicidal and pupicidal toxicity
against Musca domestica. In larvicidal bioassay, the values of LC₅₀
were 16.50, 2.03 and 6.41 mg/ml for Mo-LE, Mo-AgNPs andMo-ZnONPs,
respectively. In addition, the LC₅₀ was determined against pupae,
which were129.77, 9.604 and 17.10 mg/ml for Mo-LE, Mo-AgNPs andMo-ZnONPs,
respectively. Moreover, treatment larvae with LC₅₀'s of Mo-LE, Mo-AgNPs andMo-ZnONPs
produced a significant reduction (P<0.05) in the fecundity of female
and a highly significant reduction (P<0.01) in the egg hatchability.
The total protein content and activities of nonspecific esterases, acetylcholine esterase, and glutathione S-transferase enzymes were significantly declined after
larvae had fed on LC₅₀ Mo-AgNPs andMo-ZnONPs
treated diets as compared with the control. This study indicates that it is
feasible to use M. oleifera synthesized nanoparticles as effective candidates to develop newer and cheap
control tools for immature stages of M. domestica.