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15745

Esterase activity and detection of carboxylesterase and phosphotriesterase in female desert locust Schistocerca gregaria (Forskal) in relation to tissues and ages

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Last updated: 03 Jan 2025

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Abstract

General esterases activity in the different body
tissues of adult females of 2-day and 13-day-old Schistocerca gregaria (Forskal)
were measured spectrophotometricaly and carboxylesterase and phosphotriesterase
were characterized by electrophoresis method. Esterase activities were studied
in the tissues of brain, fore-, mid-, hindgut and ovary of the two ages in the
presence of α-naphthyl acetate as a substrate and ethylenediamine tetraacetic
acid (EDTA) and profenofos as inhibitors. Spectrophotometric analysis indicated
that the esterase activity in adult S. gregaria tissues was
significantly higher in the tissues of mid-, foregut, ovary and brain of
2-day-old females than those of 13-day-old, respectively. Meanwhile, the
activity was greater in the hindgut tissue of 13-day-old females than in
2-day-old ones. Non-denaturing polyacrylamide gel electrophoresis revealed
differences in isozyme composition. Three to ten esterase bands were detected
in the different body tissues of the two ages of adult females. These bands were
characterized as phosphotriesterase and carboxylesterase by using the two
inhibitors. Bands Est-1, Est-2, Est-3 and Est-6 were found to be
carboxylesterase in the different tissues, while band Est-4 was only detected
to be phosphotriesterase.

DOI

10.21608/eajbsa.2008.15745

Keywords

Schistocerca gregaria, carboxylesterase, phosphotriesterase, isozymes, tissues, ages, esterase activity

Authors

First Name

Eman

Last Name

Rashad

MiddleName

M.

Affiliation

Department of Zoology, Faculty of Science, Zagazig University

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Volume

1

Article Issue

2

Related Issue

3405

Issue Date

2008-12-01

Receive Date

2018-10-03

Publish Date

2008-12-01

Page Start

135

Page End

143

Print ISSN

1687-8809

Online ISSN

2090-0813

Article File

EAJBSA_Volume 1_Issue 2_Pages 135-143.pdf

PDF . 362.6kB

Link

https://eajbsa.journals.ekb.eg/article_15745.html

Detail API

https://eajbsa.journals.ekb.eg/service?article_code=15745

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12

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Original Article

Type Code

667

Publication Type

Journal

Publication Title

Egyptian Academic Journal of Biological Sciences. A, Entomology

Publication Link

https://eajbsa.journals.ekb.eg/

MainTitle

Esterase activity and detection of carboxylesterase and phosphotriesterase in female desert locust Schistocerca gregaria (Forskal) in relation to tissues and ages

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Article

Created At

22 Jan 2023