The present study was conducted to evaluate the neurotoxic effect of spinetoram on Spodoptera littoralis (Boisd.) larvae. Testing different concentrations of this green chemical compound against 4^th instar larvae showed that spinetoram is a fairly toxic with LC₅₀ (1.11 ppm). Its neurotoxicity was manifested as evident histopathological changes in the structure of neurosecretory (NSCs) and ordinary nerve cells (NCs) of suboesophageal ganglion (SOG) of this pest after treatment with LC₅₀ of this compound. Photo and electron micrographs of suboesophageal ganglion (SOG) of treated S.littoralis larvae with LC₅₀ of spinetoram showed aggregation of neurosecretory granules in the oval, triangle and irregular shaped neurosecretory cells but not in the round shaped cells. Also, the SOG of treated larvae showed an apparent vacuolization and increase in the size of cytoplasm, abundance and aggregation of mitochondria in nerve cells and all kinds of NSCs (round, irregular, triangle and oval) and the appearance of multivesicular bodies in the cytoplasm of neurosecretory cells.