Nematophagous fungi include a group of fungal antagonists to nematodes. The
nematophagous fungi have been suggested as promising candidates for biological
control of plant parasitic nematodes. Impacts of different pH levels, temperature, light
intensity, carbon and nitrogen source were tested on the growth of five nematophagus
fungi under in vitro conditions. Three isolates of Trichoderma harzianum T7, T8, and
T14, one isolate of Pochonia chlamydosporia var. chlamydosporia and one isolate of
Arthrobotrys oligospora were tested. The results of experiment indicated that the
growth of isolates of Trichoderma harzianum was maximum in pH range of 7.00-
8.00. The best growth of Pochonia chlamydosporia var. chlamydosporia and
Arthrobotrys oligospora was in 4.00-7.00. Effect of temperature differs between range
of 15-30°C, for isolates of Trichoderma harzianum was 20-25°C, Pochonia
chlamydosporia var. chlamydosporia was 15-20°C, and Arthrobotrys oligospora was
20-25°C. Maximum growth of two isolates of Trichoderma harzianum T7, T8 and
Pochonia chlamydosporia var. chlamydosporia was in darkness. Best growth of one
isolates of T. harzianum (T7) was in light and maximum growth of Arthrobotrys
oligospora and Trichoderma harzianum T14 occurred in 12 h light and 12h dark. The
best source of nitrogen for Trichoderma harzianum (T8,T14) was Nitrate potassium,
while Nitrate ammonium was the best nitrogen source for (T7).The maximum growth
of A. oligospora and P. chlamydosporia occuerd in Nitrate sodium. Between carbon
sources Arabinose are suitable for A.oligospora and Trichoderma harzianum (T7).The
maximum growth of P. chlamydosporia and T. harzianim (T14) occuerd in the use of
fructose. Best growth of T. harzianum (T8) was in Mnitole.