Male Drosophila melanogaster, like males of many other animal species, male
age and temperature affected the sperm number. Here we use Drosophila
melanogaster males of different ages and decreasing temperature on the sperm
number, to investigate the role of aging and temperature affected to sperm transferred
to female during mating. The male ages were used to show that while maximize
sperm number in young (2-3 days)and middle age (27-28 days) was contributed but
mean value of sperm number between fifty replicate was different and also the results
of one way ANOVA were significant between three age classes. Sperms after mating
soon counted from female reproductive system, the results are shown, mean value of
sperms when males exposed to cold temperature (15°c) in young and middle aged,
sperm number was around 4000, and old male aged (952-53 days) around 2000, these
results demonstrate that viability of sperm produce in males after 27- 28 days, will be
reduced more than young to middle aged.