The experiments were carried out under controlled conditions of temperatures (16, 21, 26, 32 and 37 ± 1ºC) and relative humidity 65-70 % R.H. to study the temperature effect on some biological aspects and biochemical of Earias insulana field strain when reared on okra pods. The incubation period of E. insulana eggs was 12.45 days at 16ºC. But on 37 ºC it shortened to 1.10 days. Larval duration decreased as temperature increased when reared on okra pods. Similar trend was recorded for pupal duration. Generation period lasted 38.27 days on 21 ºC but it shortened to 17.4 days on 32 ºC. The thresholds of development (T_o) were 15.95, 14.41, 13.32, 17.63 and 12.85 ºC for eggs, larvae, pupae, pre- oviposition and generation period of E. insulana, respectively. While, the average of thermal units required to complete the developmental stages were 23.15, 131.33, 90.79, 20.83 and 346.19 DD̉s, respectively, when reared on okra pods. The total eggs laid by female high significantly affected by all temperatures. The highest number of eggs laid at 26 followed by 21ºCbinge 273.66 and 208.00 eggs/female. The low and high temperatures caused the highest reduction in total lipid and protein. The reduction in total lipid and protein caused reduction in the fecundity and fertility of E. insulana adult female.