The insect infestation of stored grain and their products is a serious problem over the world since it reduces crop yields, causes contamination for stored products, damages seed germs, carries diseases that effect plants, peoples, animals, and changes the quality of stored products. Six compounds, one organophosphorus, pirimiphose methyl, two biocides; ivomic and Bacillus thuringiensis, one mineral oil (KZ), one insect growth regulator, cascade and one organic acid, formic acid were evaluated against Trogoderma granarium by exposure to treated media and other two ones were layer manner. Based on LC_50,s values, data indicated that primiphos-methyl was the most toxic compound with LC₅₀ of 0.135 ug/g, while the least toxic one was the mineral oil with LC₅₀ value of 4300 ug/g grain at 24 h post-treatment. The efficacy of the biocide ivomic followed the effect of pirimiphos-methyl. Data obtained cleared that cascade had a higher toxic effects on the tested insect than the mineral oil formic acid. Batches of grains (wheat grain) were mixed using the three mentioned manners. Based on the behavior of stored product insects which quickly move between grains where contact with the treated media. Results obtained showed that half and one third of dose had more percent of mortality than the expected values. The down position of treated layer indicated the most effect compared to the other two layers (middle and surface).