The fertilization have an important role in the abundance and population of different mite species associated with different agricultural crops. As a result of experiment and investigation on the population of mites inhabiting soil underneath Cotton (Gossypium barbadense L.) in Giza Governorate, (Kerdasa region) during April to September 2014 and 2015 to study the effect of applying lay soil with the rice straw as organic wastes with the inoculation with Humic acid both separately or together on the population of different soil mites. The obtained results revealed the presence of 17 different mite species belonging to 11 families related to four suborders, Prostigmata (Actinedida), Mesostigmata (Gamasida), Astigmata (Acaridida) and Cryptostigmata (Oribatida) associated with soil of cottonplants treated with different types of fertilizers. The percentage of collected four soil mites suborders, the actinedids were higher in the abundance inhabiting cotton plantssoil in comparison with other three collected mite groups, followed by oribatid mites, followed by acaridid mites but the gamasid mites abundance was the lower category in all three tested methods of fertilization. The obtained data demonstrated that the mean number of collected mites in case of using recommended + humic acid fertilization method was higher than those recorded in recommended only and in case of recommended + humic + rice collectively. The study also indicated that the collected different mites during 2015 cultivated season were higher in number than those obtained during the first season 2014. The obtained data also demonstrated that September 2014 was the most study period harbored collected mites in all different methods of fertilization.