Spiders were sampled from organically and conventionally farmed Chamomile, Matricaria chamomilla and Chrysanthemum, Calendula officinalis in two successive growing seasons 2014/2015 and 2015/2016 in Fayoum region. Spiders were collected by ground pitfall traps. Seventeen species of spiders were recorded. Most of collected species belong to the family Lycosidae, with especially high captures of Honga sp., Pardosa sp., and Wadicosa fidelis. The Gnaphosidae was represented by Micaria dives and Zelotes laetus, although the samples were largely dominated by the presence of Steatoda erigoniformis, Theridiidae. Results showed that more spider population, and a greater number of spider species, were captured from organic than from conventional fields.