A little attention has been given on molecular identification of thrips (Insecta: Thysanoptera) in Egypt. Thus, the primers ITS4A, 28S rRNA, 18S rRNA and the primer pairs COI-1 + COI-2 + COI-3 were used to identify the three dominant thrips species (Haplothrips cahirensis karny; Dendrothrips eremicola Priesener and Hydatothrips kassimanus Priesener). The samples were collected from a private olive grove during 2013 and 2014 at Ismailia governorate, Egypt. DNA sequence data for 28S rRNA gene showed apositiveresponseto H. cahirensis and D. eremicola only, while 18S rRNA reacted with all species. ITS4A and COI-1 + COI-2 + COI-3 showed a negative response to the studied species. DNA sequences of the species based on 18S rRNA were recorded in the Gene Bank. The phylogenetic tree of the three thrips species was given also.