This work was conducted to determine the effect of two different fungi i.e. Aspergillus flavus and Fusarium oxysporum as food sources on the biology of the mesostigmatid fungivorous mite Proctolaelaps pygmaeus (Ascidae) at different laboratory conditions (15, 25 and 35+2°C and 75+5% R.H.). Also, the effect of yeast and Fusarium oxysporum on the biological aspects of the astigmatid mite, Glycyphagus ornatus (Glycyphagidae) was determined at different laboratory conditions (15, 25 and 35°C and 75% R.H.). Theincubation period,life cycle, longevity and life span of both females and males and the fecundity (number of eggs) of the females of different tested fungivorous mites weresignificantly differed according to the different experiment conditions (diets and temperatures). The developmental periods of P. pygmaeus and G. ornatus were faster for the male members than females and 15°C increased these periods, than 35°C. The number of deposited eggs by females of the both P. pygmaeus and G. ornatus differed according to the kind of food source and used temperature, as the number of P. pygmaeus deposited eggs increased when the individuals fed on F. oxysporum at 35°C than on A. flavus at 15 and 25°C. Also, the number of deposited eggs by females of G. ornatus was obviously increased when females reared on yeast at 25°Cthan any other diets and temperatures.