Toxic influence of Chlorpyrifos, Lambda-Cyhalothrin, Methomyl and Spinosad against lab and field moths of the pink bollworm, Pectinophora gossypiella (Saund.) were tested under Laboratory conditions. Methomyl had the superior toxic effect followed by Lambda-Cyhalothrin, Chlorpyrifos and Spinosad (LC50 = 1.16, 2.45, 4.01 and 20.75 ppm respectively) to Lab insects. The field insects from Menoufia Governorate were more tolerant to toxic effect of the tested insecticides especially chlorpyrifos than Bani-Suif Governorate insects. A high significant increase of enzyme activity was determined in Glutathione-S-Transferase (GST) than MFO Cytochrome P450 (PCMAN-demethylase) of Field moths than lab ones. While the depletion in enzyme activity was presented in Acetylcholinesterase enzyme of field insects. These changes in enzymes activity of the field insects may be correlated with their tolerance ability to toxic effect of the tested insecticides.