Field studies were carried out to evaluate seven cotton varieties i.e. Giza 86, Giza 87, Giza 88, Giza 92, Giza 93, Giza 94 and Giza 96 for their liability to two spotted red mite, Tetranychus urticae Koch infestation and its associated predacious phytoseiidae mite, Euseius scutalis (Athias-Henriot)during 2014 and 2015 cotton growing seasons at two different Governorates; Sharkia and Kafr El Sheikh. Giza 86 was the most susceptible variety in Kafr El Sheikh during the two successive seasons, while in Sharkia Governorate, Giza 92 and Giza 96 were the most response to T. urticae infestation during 2014 and 2015 seasons, respectively. Results showed the populations of phytoseiidae, E. scutalisv aried according to the tested varieties and growing seasons. G. 94 proved the highest significant yield in Kafr El Sheikh during the two successive seasons, recording 7.57±0.47 and 13.07±1.20 kintar during 2014 and 2015 seasons, respectively. While recorded in Sharkia (7.86±0.25 and11.60±0.20) in both 2014 and 2015 seasons. Moreover, temperature and humidity played a key role in the infestation abundance of T. urticae in tested cotton varieties. A positive relationship was found between mite infestation and both nitrogen and protein contents in tested cotton leaves. The varieties Giza 96 and Giza 86 recorded the highest significant amounts of both total protein and nitrogen manifested the highest significant infestation of mites in both Sharkia and Kafr El Sheikh, respectively.