The house fly, Musca domestica L. is considered as a notorious pest by medical, veterinary, and public health professionals worldwide. This study was carried out to evaluate the effect of different entomopathogenic nematode (EPNs) strains against the 3^rd larval instar and pupal stage of the house fly. Two of them were native strains (Heterorhabditis indica and Steinernema carpocapsae) and the others were imported strains Heterorhabditis bacteriophora and Steinernema glaseri. The results indicated that the mortality of all tested stages of M. domestica were significantly influenced by the infective juveniles (IJs) concentrations and the exposure time where the maximum mortality was recorded 72 hrs. post-treatment and the larvae were more susceptible to nematodes pathogenicity than the pupae. Also, the Heterorhabditis nematodes H. baceriophora and H. indica appeared to be more effective than the Steinernema species, and H. bacteriophora (Hb88) was the most promising strain in management of both larvae and pupae causing the lower LC₅₀ and LC₉₅ (320.4 and 1987IJs/ml) for larvae and (1414.6 and 2664.4 IJs/ml) for pupae, respectively.