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11691

Evaluation of the Role of Irradiated, Culex pipiens , Mosquito (Diptera; Culicidae) in the Transmission of Hepatitis C Virus ( HCV)

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Last updated: 22 Jan 2023

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Abstract

In the present study, gamma radiation effects on the reproductive potential of the mosquito, Culex  pipiens and its role in the transmission of the Hepatitis C Virus (HCV) were investigated. The susceptibility of female, Culex pipiens mosquitoes to gamma irradiation was carried out by exposed full grown pupae to doses 0, 20, 40, 60 Gy. The lethal doses were calculated, as the doses of gamma radiation increased, a progressive increase in the non- hatched eggs percentage. The viral load at mouth parts, in mid-gut and salivary gland, was detected in the irradiated females with LD75 (60 Gy) and non-irradiated using RT-PCR relatively at time 60 min., 5 and 13 days and tested in the mentioned position. Viral load in irradiated Culex pipiens that fed on an infected blood with a viral load 1.2 x 106IU/ml %was decreased by time from 6.0782 x 104 IU/ML% at zero time into 2.399 x 103 IU/ML% after 60 min. post-infection at the mouth parts. Also, the viral load decreased by time in the mid-gut from 2.63575 x 105  IU/ML% at zero time into 3.969 x 103  IU/ML%  after 5 days post-infection, while  HCV was not detected in the salivary glands. The current results indicated that the mechanical transmission through mouth parts in irradiated and non-irradiated Culex pipiens mosquitoes is plausible while the biological transmission did not occur. 

DOI

10.21608/eajb.2018.11691

Keywords

Mosquitoes, Radiation, HCV, Transmission

Authors

First Name

Mostafa

Last Name

Hassan

MiddleName

I.

Affiliation

Department of Zoology and Entomology, Faculty of Science , Al-Azhar University, Nasr City, Cairo, Egypt

Email

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City

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Orcid

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First Name

Aly,

Last Name

Mohamed

MiddleName

F.

Affiliation

VACSERA Holding Company for production of vaccines, sera and drugs, Giza-Egypt

Email

-

City

-

Orcid

-

First Name

Kotb

Last Name

Hammad

MiddleName

M.

Affiliation

Department of Zoology and Entomology, Faculty of Science , Al-Azhar University, Nasr City, Cairo, Egypt

Email

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City

-

Orcid

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First Name

Gabarty

Last Name

A.

MiddleName

-

Affiliation

Natural Products Department, National Center for Radiation Research and Technology, Atomic Energy Authority, Cairo, Egypt.

Email

ahlamalgabarty@yahoo.com

City

-

Orcid

-

First Name

Tharwat

Last Name

Selim

MiddleName

A.

Affiliation

Department of Zoology and Entomology, Faculty of Science , Al-Azhar University, Nasr City, Cairo, Egypt

Email

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City

-

Orcid

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Volume

11

Article Issue

3

Related Issue

2145

Issue Date

2018-06-01

Receive Date

2018-05-24

Publish Date

2018-06-01

Page Start

139

Page End

148

Print ISSN

1687-8809

Online ISSN

2090-0813

Article File

EAJBSA_Volume 11_Issue 3_Pages 139-148.pdf

PDF . 608.9kB

Link

https://eajbsa.journals.ekb.eg/article_11691.html

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https://eajbsa.journals.ekb.eg/service?article_code=11691

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Original Article

Type Code

667

Publication Type

Journal

Publication Title

Egyptian Academic Journal of Biological Sciences. A, Entomology

Publication Link

https://eajbsa.journals.ekb.eg/

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Article

Created At

22 Jan 2023