Introduction: In fuel stations, gasoline induces a wide range of toxicological effects on body tissues and biochemical changes, which pose grave health risks to humanity. Aim: This study aims to assess the effects of gasoline on hematological parameters, liver and kidney functions among sample of fuel station workers in Sohag governorate, Egypt. Methods: A cross-sectional case control study was done on 100 males working in fuel stations and 50 males didn't work in fuel stations in Sohag governorate, in the duration of one year from first of April 2019 to end of March 2020. Each participant was interviewed with a detailed questionnaire and CBC, liver and kidney function tests were done. Results: The study revealed statistically high significant differences between the studied and the control groups as regards RBCs count and the levels of ALT, AST, total protein and urea. There was a statistically significant effect of smoking on RBCs count, WBCs count, AST level, creatinine and urea level of the studied group. There was statistically significant positive correlation between parameters of CBC, liver and kidney function tests and ages of the workers of fuel station and there was only positive correlation between ALT level and the duration of work. Conclusion: this study concluded that exposure to gasoline associated with significant decrease of RBCs count and total protein level and significant increase of ALT, AST and urea levels among workers in fuel stations and these effects increase with advancing in age and most of them get worse with increased duration of work at fuel stations and with smoking. Recommendations: Our study recommends improving working conditions and using natural gas for cars as an alternative to gasoline