Beta
ManageSearchChatDiscover
179173

Optimization of antifungal activity by Bacillus subtilis isolate CCASU 2021-4 using Response Surface Methodology

Article

Last updated: 03 Jan 2025

OverviewSimilar Items

Subjects

-

Tags

-

Abstract

Fungal infections represent an enormous load on the public especially with the development of resistance to the most currently used antifungal drugs in practice. In the present work, a bacterial isolate coded A3 was recovered from soil and was shown to express antagonistic activity against Candida (C.) albicans ATCC 10231. This isolate was identified as Bacillus (B.) subtilis subsp. spizizenii isolate CCASU 2021-4 using 16S ribosomal RNA sequencing. D-optimal design from response surface methodology (RSM) was used to optimize the environmental variables affecting antifungal activity of the respective isolate. The optimum conditions were a temperature of 30 °C, a pH of 8 and an inoculum size of 5 x 107 cfu/ml, resulting in an enhancement in the antifungal activity by 1.2 fold. This is the first report, to the best of our knowledge, on an antifungal activity from B. subtilis subsp. spizizenii culture broth against human fungal pathogens along with its optimization through RSM.

DOI

10.21608/aps.2021.80383.1063

Keywords

Antifungal, Optimization, D optimal design, Bacillus subtilis sp, Response surface methodology

Authors

First Name

Ghadir

Last Name

El-Housseiny

MiddleName

-

Affiliation

Department of Microbiology and Immunology, Faculty of Pharmacy, Ain Shams University, Cairo 11566, Egypt

Email

ghadir.elhossaieny@pharma.asu.edu.eg

City

-

Orcid

-

First Name

Gina

Last Name

Shams

MiddleName

-

Affiliation

Faculty of Pharmacy, Ain Shams University, Cairo 11566, Egypt

Email

ginashams97@yahoo.com

City

-

Orcid

-

First Name

Zeina

Last Name

Ghobashi

MiddleName

-

Affiliation

Faculty of Pharmacy, Ain Shams University, Cairo 11566, Egypt

Email

zeinaghobashi@gmail.com

City

-

Orcid

-

First Name

Rawan

Last Name

Mamdouh

MiddleName

-

Affiliation

Faculty of Pharmacy, Ain Shams University, Cairo 11566, Egypt

Email

rawan.mamdouh@pharm.asu.edu.eg

City

-

Orcid

-

First Name

Israa

Last Name

Almaqsod

MiddleName

-

Affiliation

Department of Microbiology and Immunology, Faculty of Pharmacy, Ain Shams University, Cairo 11566, Egypt

Email

israa.m.almaqsod@pharma.asu.edu.eg

City

-

Orcid

-

First Name

Sarra

Last Name

Saleh

MiddleName

-

Affiliation

Department of Microbiology and Immunology, Faculty of Pharmacy, Ain Shams University, Cairo 11566, Egypt

Email

sarradeif@pharma.asu.edu.eg

City

-

Orcid

-

Volume

5

Article Issue

1

Related Issue

25600

Issue Date

2021-06-01

Receive Date

2021-06-12

Publish Date

2021-06-01

Page Start

171

Page End

183

Print ISSN

2356-8380

Online ISSN

2356-8399

Article File

APS_Volume 5_Issue 1_Pages 171-183.pdf

PDF . 1.2MB

Link

https://aps.journals.ekb.eg/article_179173.html

Detail API

https://aps.journals.ekb.eg/service?article_code=179173

Order

13

Type

Original Article

Type Code

657

Publication Type

Journal

Publication Title

Archives of Pharmaceutical Sciences Ain Shams University

Publication Link

https://aps.journals.ekb.eg/

MainTitle

Optimization of antifungal activity by Bacillus subtilis isolate CCASU 2021-4 using Response Surface Methodology

Details

Type

Article

Created At

22 Jan 2023