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177713

Identification of potential quorum quenching compounds in Brassica oleracea var. capitata against MDR Pseudomonas aeruginosa and Escherichia coli clinical isolates

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Last updated: 22 Jan 2023

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Abstract

Over the last decades, the development of microbial resistance has become an alarming situation. This has urged the search for new antimicrobial strategies. In this context, two Brassicaceae edible plants; Brassica oleracea var. capitata (cabbage) and Brassica rapa subsp. rapa (Turnip) were assessed for their antimicrobial activity against P. aeruginosa and E. coli clinical isolates. Antibiogram analysis was done according to the CLSI 2019 guidelines and proved that both P. aeruginosa and E. coli clinical isolates were multidrug resistant. A green extraction methodology – assisted by microwave and ultrasound- was used to prepare the aqueous extracts. Determination of minimum inhibitory concentration (MIC) of the extracts was also carried out according to the CLSI guidelines. At sub-MIC concentration, cabbage extract showed promising results in the inhibition of quorum sensing mediated virulence determinants of P. aeruginosa. Highest reduction was observed in pyocyanin and rhamnolipid production. Chemical profiling via UPLC-ESI-MS analysis of cabbage extract revealed the presence of different glucosinolates together with iberin and sulforaphane. The in silico docking study was conducted and revealed the ability of sulforaphane and iberin to bind to LasR regulator responsible for quorum sensing in P. aeruginosa. These compounds thus represent potential candidates that can be developed into novel antimicrobial infection control tools.

DOI

10.21608/aps.2021.76856.1062

Keywords

Quorum sensing, Iberin, Sulforaphane, cabbage, resistance, docking

Authors

First Name

Masarra

Last Name

Sakr

MiddleName

-

Affiliation

Department of Microbiology and Immunology, Faculty of Pharmacy, Ain Shams University, Cairo 11566, Egypt

Email

masara.mohamed@pharm.asu.edu.eg

City

Cairo

Orcid

0000-0001-9906-9435

First Name

Nehal

Last Name

Ibrahim

MiddleName

-

Affiliation

Department of Pharmacognosy, Faculty of Pharmacy, Ain Shams University, Cairo 11566, Egypt

Email

nehal.sabry@pharma.asu.edu.eg

City

-

Orcid

0000-0002-6526-298X

First Name

Sandy

Last Name

Ali

MiddleName

-

Affiliation

Faculty of Pharmacy, Ain Shams University, Cairo 11566, Egypt

Email

sandy.othman@pharma.asu.edu.eg

City

-

Orcid

-

First Name

Nouran

Last Name

Alzahaby

MiddleName

-

Affiliation

Faculty of Pharmacy, Ain Shams University, Cairo 11566, Egypt

Email

nouran.amgad@pharma.asu.edu.eg

City

-

Orcid

-

First Name

Aya

Last Name

Omar

MiddleName

-

Affiliation

Faculty of Pharmacy, Ain Shams University, Cairo 11566, Egypt

Email

aya.abdelbaky@pharma.asu.edu.eg

City

-

Orcid

-

First Name

Walaa

Last Name

Khairy

MiddleName

-

Affiliation

Faculty of Pharmacy, Ain Shams University, Cairo 11566, Egypt

Email

walaa.othman@pharma.asu.edu.eg

City

-

Orcid

-

First Name

Bassant

Last Name

Zohdy

MiddleName

-

Affiliation

Faculty of Pharmacy, Ain Shams University, Cairo 11566, Egypt

Email

bassant.abdelmonem@pharma.asu.edu.eg

City

-

Orcid

-

First Name

Omar

Last Name

Qassem

MiddleName

-

Affiliation

Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Ain Shams University, Cairo 11566, Egypt

Email

omar.m.hasan@pharma.asu.edu.eg

City

-

Orcid

-

First Name

Sarra

Last Name

Saleh

MiddleName

-

Affiliation

Department of Microbiology and Immunology, Faculty of Pharmacy, Ain Shams University, Cairo 11566, Egypt

Email

sarradeif@pharma.asu.edu.eg

City

-

Orcid

-

Volume

5

Article Issue

1

Related Issue

25600

Issue Date

2021-06-01

Receive Date

2021-05-23

Publish Date

2021-06-01

Page Start

128

Page End

142

Print ISSN

2356-8380

Online ISSN

2356-8399

Article File

APS_Volume 5_Issue 1_Pages 128-142.pdf

PDF . 925.8kB

Link

https://aps.journals.ekb.eg/article_177713.html

Detail API

https://aps.journals.ekb.eg/service?article_code=177713

Order

10

Type

Original Article

Type Code

657

Publication Type

Journal

Publication Title

Archives of Pharmaceutical Sciences Ain Shams University

Publication Link

https://aps.journals.ekb.eg/

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Details

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Article

Created At

22 Jan 2023