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Evaluation of antimicrobial activity and in vitro safety of the methanolic extract of Streptomyces manipurensis soil isolate H21 for potential industrial applications

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Last updated: 22 Jan 2023

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Abstract

Streptomyces manipurensis isolate H21 was recovered from a soil sample in Cairo, Egypt. Cell-free culture supernatant of Streptomyces manipurensis isolate H21 previously showed antifungal and broad-spectrum antibacterial activity against some Gram-positive, Gram-negative, MDR, and ESBL producer pathogens as well as some reference strains. The present study aimed at investigating antimicrobial and cytotoxic activities of its crude methanolic extract. The antibacterial activity of the crude methanolic extract was determined using broth-dilution method against Staphylococcus aureus ATCC 43300, Klebsiella oxytoca ATCC 700324, Klebsiella pneumoniae ATCC 700603, Klebsiella pneumoniae ATCC BAA-1705 and 2 MDR uropathogens. The minimum inhibitory concentrations (MICs) and the minimum bactericidal concentrations (MBCs) of the crude extract against the tested bacteria were in the range of 5-10 mg/mL and 10 mg/mL, respectively. The results revealed that the CD50 value of the extract was 1.17 mg/mL, against Caco-2 cell line, indicating in vitro safety and low cytotoxicity. Accordingly, Streptomyces manipurensis isolate H21 would be an excellent source of relatively safe and potent antibacterial agent.

DOI

10.21608/aps.2019.20201

Keywords

Streptomyces manipurensis, agar well diffusion, MIC, MBC, MTT assay

Authors

First Name

Yomna

Last Name

Elkholy

MiddleName

N.

Affiliation

Microbiology and Immunology Department, Faculty of Pharmacy, Ain Shams University, Abbassia, Cairo11566, Egypt

Email

yomnanagy@gmail.com

City

-

Orcid

-

First Name

Walid

Last Name

Elkhatib

MiddleName

Faisal

Affiliation

Department of Microbiology and Immunology, Faculty of Pharmacy, Ain Shams University, African Union Organization St., Abbassia 11566, Cairo, Egypt

Email

walid-elkhatib@pharma.asu.edu.eg

City

-

Orcid

0000-0001-5815-3200

First Name

Khaled

Last Name

Aboshanab

MiddleName

M.

Affiliation

Department of Microbiology and Immunology, Faculty of Pharmacy, Ain Shams University, Cairo, Egypt

Email

aboshanab2012@pharma.asu.edu.eg

City

Cairo

Orcid

0000-0002-7608-850X

First Name

Mohammad

Last Name

Aboulwafa

MiddleName

-

Affiliation

Department of Microbiology and Immunology, Faculty of Pharmacy, Ain Shams University

Email

maboulwafa@yahoo.com

City

-

Orcid

-

First Name

Nadia

Last Name

Hassouna

MiddleName

A.

Affiliation

Microbiology and Immunology Department, Faculty of Pharmacy, Ain Shams University, Abbassia, Cairo11566, Egypt

Email

nadiahassouna46@gmail.com

City

-

Orcid

-

Volume

3

Article Issue

1

Related Issue

2131

Issue Date

2019-01-01

Receive Date

2019-03-30

Publish Date

2019-06-01

Page Start

1

Page End

10

Print ISSN

2356-8380

Online ISSN

2356-8399

Article File

APS_Volume 3_Issue 1_Pages 1-10.pdf

PDF . 762.7kB

Link

https://aps.journals.ekb.eg/article_29011.html

Detail API

https://aps.journals.ekb.eg/service?article_code=29011

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Original Article

Type Code

657

Publication Type

Journal

Publication Title

Archives of Pharmaceutical Sciences Ain Shams University

Publication Link

https://aps.journals.ekb.eg/

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Article

Created At

22 Jan 2023