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18733

Biotransformation of caffeic acid into a promising biologically active metabolite by Candida albicans isolate CI-24

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Last updated: 03 Jan 2025

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Abstract

In this study, a total number of 92 clinical isolates of Candida species was used to test their ability to transform caffeic acid using the two-stage fermentation protocol. The success of the biotransformation process was confirmed by TLC autography method and nuclear magnetic resonance analysis. The obtained chromatograms showed that 7 isolates could perform caffeic acid biotransformation. The biological activities (antibacterial, antifungal, antiviral, and cytotoxic activities) of the extracts of the selected isolates were determined. According to the obtained results, the Candida isolate CI-24 had the most promising biotransformation ability. The methanolic extract of the respective isolate showed a promising anticancer activity against Caco-2 cell line and a potential antibacterial activity against Staphylococcus aureus ATCC 25923. The isolate was genetically identified as Candida albicans (Accession number MH356583) using 28S rRNA sequencing. As determined by NMR and LC-MS analysis, caffeic acid was transformed by Candida albicans strain CI-24 into para-hydroxybenzoic acid.

DOI

10.21608/aps.2018.18733

Keywords

Biotransformation, Caffeic acid, Candida albicans, Antibacterial, Cytotoxicity

Authors

First Name

Raghda

Last Name

Singab

MiddleName

A.

Affiliation

Department of Microbiology and Immunology, Faculty of Pharmacy, Ain Shams University, Cairo 11566, Egypt

Email

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City

-

Orcid

-

First Name

Ahmed

Last Name

Elissawy

MiddleName

M.

Affiliation

Department of Pharmacognosy, Faculty of Pharmacy, Ain Shams University, Cairo 11566, Egypt

Email

aelissawy@pharma.asu.edu.eg

City

-

Orcid

-

First Name

Walid

Last Name

Elkhatib

MiddleName

Faisal

Affiliation

Department of Microbiology and Immunology, Faculty of Pharmacy, Ain Shams University, African Union Organization St., Abbassia 11566, Cairo, Egypt

Email

walid-elkhatib@pharma.asu.edu.eg

City

-

Orcid

0000-0001-5815-3200

First Name

Mahmoud

Last Name

Yassien

MiddleName

A.

Affiliation

Department of Microbiology and Immunology, Faculty of Pharmacy, Ain Shams University, Cairo 11566, Egypt

Email

mahmoud.yassien@pharma.asu.edu.eg

City

-

Orcid

-

First Name

Nadia

Last Name

Hassouna

MiddleName

A.

Affiliation

Department of Microbiology and Immunology, Faculty of Pharmacy, Ain Shams University, Cairo 11566, Egypt

Email

-

City

-

Orcid

-

Volume

2

Article Issue

1

Related Issue

2032

Issue Date

2018-01-01

Receive Date

2018-03-04

Publish Date

2018-06-01

Page Start

37

Page End

46

Print ISSN

2356-8380

Online ISSN

2356-8399

Article File

APS_Volume 2_Issue 1_Pages 37-46.pdf

PDF . 824.7kB

Link

https://aps.journals.ekb.eg/article_18733.html

Detail API

https://aps.journals.ekb.eg/service?article_code=18733

Order

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Type

Original Article

Type Code

657

Publication Type

Journal

Publication Title

Archives of Pharmaceutical Sciences Ain Shams University

Publication Link

https://aps.journals.ekb.eg/

MainTitle

Biotransformation of caffeic acid into a promising biologically active metabolite by Candida albicans isolate CI-24

Details

Type

Article

Created At

22 Jan 2023