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275796

Molecular Identification of Ladybird Beetles (Coccinella: Coccinellidae) Using DNA Barcodes

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Last updated: 22 Jan 2023

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Abstract

DNA barcoding is a molecular genetics technique commonly applied for species identification based on the cytochrome c oxidase subunit I (COI) gene located on the mitochondrial DNA genome. Based on our analysis, the DNA barcodes were generated from 16 specimen of Coccinella (family Coccinellidae). All the samples were collected from diverse sites in Egypt and Libya. Morphological traits based on the number of dorsal spots, DNA extraction, PCR amplification of COI, sequencing, nucleotide BLAST, and phylogenetics analyses were used to classify the specimens. A phylogenetic tree was constructed using the maximum likelihood method, including 46 COI sequences (the study and NCBI sequences). Three Coccinella clusters were defined and classified as Coccinella septempunctata, Coccinella novemnotata, and Coccinella undecimpunctata each species clustered into a unique branch. DNA barcodes discriminated clearly against the analyzed species. This study validated the COI efficiency as a marker for DNA barcoding of insects (Coccinella, family Coccinellidae).

DOI

10.21608/jsrs.2022.275796

Keywords

Coccinella beetles, COI gene, molecular genetics, species ID, Phylogeny

Authors

First Name

Hanan

Last Name

Abdalla

MiddleName

Altaief

Affiliation

Zoology Department, Collage of Women, Ain Shams University, Cairo, Egypt.

Email

hanan.altyf@gmail.com

City

Cairo

Orcid

-

First Name

Yosur

Last Name

Gamal Fiteha

MiddleName

-

Affiliation

Zoology Department, Faculty of Women, Ain Shams University, Cairo, Egypt., Genetics Department, Faculty of Agriculture, Ain shams University, Cairo, Egypt.

Email

-

City

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Orcid

-

First Name

Mohamed

Last Name

Abdel-Salam Rashed

MiddleName

-

Affiliation

Genetics Department, Faculty of Agriculture, Ain shams University, Cairo, Egypt.

Email

-

City

-

Orcid

-

First Name

Ramadan

Last Name

Mohamed Ali

MiddleName

-

Affiliation

Zoology Department, Faculty of Women, Ain Shams University, Cairo, Egypt.

Email

ramadanali27@gmail.com

City

-

Orcid

-

First Name

Aya

Last Name

Mohamed Abdel-Salam Hammad

MiddleName

-

Affiliation

Zoology Department, Faculty of Women, Ain Shams University, Cairo, Egypt.

Email

-

City

-

Orcid

-

First Name

Ahmed

Last Name

Saber Mohamed Bream

MiddleName

-

Affiliation

Entomology Dept., Faculty of Sciences, Al-Azhar University, Cairo, Egypt.

Email

-

City

-

Orcid

-

First Name

Mahmoud

Last Name

Magdy

MiddleName

-

Affiliation

Genetics Department, Faculty of Agriculture, Ain shams University, Cairo, Egypt.

Email

-

City

-

Orcid

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Volume

39

Article Issue

2

Related Issue

38373

Issue Date

2022-12-01

Receive Date

2022-12-24

Publish Date

2022-12-01

Page Start

179

Page End

193

Print ISSN

2356-8364

Online ISSN

2356-8372

Article File

JSRS_Volume 39_Issue 2_Pages 179-193.pdf

PDF . 1.7MB

Link

https://jsrs.journals.ekb.eg/article_275796.html

Detail API

https://jsrs.journals.ekb.eg/service?article_code=275796

Order

275,796

Type

Original Article

Type Code

656

Publication Type

Journal

Publication Title

Journal of Scientific Research in Science

Publication Link

https://jsrs.journals.ekb.eg/

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Details

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Article

Created At

22 Jan 2023