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The Effects of Different Endoglycosidases on Erythrocyte CD44, CD47 and CD147 Purified by Affinity Chromatography

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Last updated: 24 Dec 2024

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Abstract

Endoglycosidases are enzymes with the ability to cleave certain N-and O- linked glycans attached to glycoproteins. The biochemical structure of some erythrocyte glycoproteins attached carbohydrate chains are not elucidate yet. The aim of this study is to investigate the effects of different endoglycosidases on three purified erythrocyte glycoproteins, CD147, CD47 and CD44, which will also provide clues about the structure of their N-linked glycans. Peptide-N-glycosidase F (PNGaseF), Endo-β-N-acetylglucosaminidase F1 (EndoF1), endo-β-N-acetylglucosaminidase H (EndoH) or Endo-β-galactosidase (Endoβ) was used to digest the glycoproteins purified by antibody high-affinity chromatography, and the results were analysed by sodium dodecyl sulfate polyacrylamide gel electrophoresis. CD147, CD47 and CD44 were purified effectively by BRIC235, BRIC126 and MA103, respectively. PNGaseF caused a significant variation in the electrophoretic mobility of the glycoproteins, while no remarkable effects of Endoβ were detected. EndoH caused a reduction in the molecular weight of CD47 and CD44, although it did not appear to cleave all the glycans. In contrast, EndoH cleaved almost all glycans from CD147. Our results indicate that most of the N-linked glycans of CD147 and CD47 are high-mannose and hybrid types. The findings suggest that a significant amount of CD44 N-glycans are from complex oligosaccharides.  

DOI

10.21608/jsrs.2019.77047

Keywords

CD44, CD47, CD147, endoglycosidase, N-glycan, erythrocyte

Authors

First Name

Mohrah

Last Name

Alashaikh

MiddleName

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Affiliation

Clinical Laboratory Sciences, College of Applied Medical Sciences, King Saud University, Riyadh, Kingdom of Saudi Arabia.

Email

malalshaikh@ksu.edu.sa

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Orcid

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Volume

36

Article Issue

1

Related Issue

5321

Issue Date

2019-04-01

Receive Date

2020-03-11

Publish Date

2019-04-01

Page Start

494

Page End

511

Print ISSN

2356-8364

Online ISSN

2356-8372

Article File

JSRS_Volume 36_Issue 1_Pages 494-511.pdf

PDF . 734.6kB

Link

https://jsrs.journals.ekb.eg/article_77047.html

Detail API

https://jsrs.journals.ekb.eg/service?article_code=77047

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32

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Original Article

Type Code

656

Publication Type

Journal

Publication Title

Journal of Scientific Research in Science

Publication Link

https://jsrs.journals.ekb.eg/

MainTitle

The Effects of Different Endoglycosidases on Erythrocyte CD44, CD47 and CD147 Purified by Affinity Chromatography

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Type

Article

Created At

22 Jan 2023