The study objective evaluate the effect of Nitrobein biofertilizer on plant growth parameters and cytogenetic alterations through the mitotic and meiotic divisions of Vicia faba after treated with different doses (D, 2Dand 4D) compared with control. The variation in the content of the genetic material through G1, S, G2 at cell cycle had a proportion of cells entering the cell cycle at interphase as an indicator for cell proliferation and percentage of mitotic index  by using cellular image analysis based on the relationship between DNA as Eeuchromatin and  Heterochromatin and the Feulgen stain intensity, added  to followed the chromosome behavior through  M phases from cell cycle at mitotic and meiotic divisions, where the recorded results had a proportional relationship with biofertilizer doses (D,2Dand 4D) compared with control accompanied with the mutagenic effects as a results of chromosome aberration at metaphase stage represented by bridges, fragment  and nondisjunction chromosome at anaphase based on the end-to-end fusions between sister chromosomes or sister chromatids end joining as a result telomere dysfunction and anomalies chromosomal types was permanent where its continued from somatic cells to gametic cells in meiotic division. On the other hand, there is a significant increase in growth parameters after treated with biofertilizer doses