A bioactive glass/agarose/gelatin nanocomposite scaffold was prepared for bone engineering application. The scaffold was characterized by Scaning Electron Microscopy (SEM), Fourier infrared spectra (FTIR), andThermogravimetric Aanalysis(TGA). SEM micrographs showed that the scaffold had a well-interconnected macroporous structure. TGA indicated that the glass content in the scaffold was 57.12% which is close to the predecided one (60 wt%). The porosity percentage of the scaffold was measured by mercury porosimetry which showed that the scaffold has high porosity percentage (71.30%). In vitro bioactivity evaluation was carried out by immersion of the scaffold in simulated body fluid (SBF) for 2 and 4 weeks. Results showed that the scaffold was able to induce an apatite layer on its surface as verified by SEM, FTIR and Thin Film X-ray (TXRD).