The present study was carried out to investigate the efficiency of barley or malted barley in ameliorating lipid profile and oxidative stress alteration caused by high fat, high cholesterol diet (HFHC).Adult male albino rats were divided into six groups; each group consisted of 12 rats. Group (1) Served as control, fed on balanced diet (without treatment);groups (2) and (3), rats were fed on balanced diet containing 10 %barley and malted barley respectively, group (4),rats were fed on HFHC (50 g lard+10 g cholesterol/kg diet) to induce hypercholesterolemia. Groups (5) and (6), rats were fed on HFHC contains 10% barley and malted barley respectively. Resultsreflected a significant depletion in all parameters of lipids profile and the percentage of change reached  22.04 %  24.87%, 25.19 %, 40.34 % , 34.11 % , 54.37 % , 54.89 % for Total lipid (TL), Total cholesterol(TC) ,Triacylglycerols (TAG) , Low density lipoprotein cholesterol (LDL-C) ,Very low density lipoprotein cholesterol (VLDL-C) ,Atherogenic index(AI) and Risk factor ratio (RF) respectively when compared with HFHC group treated with barley with an exception of high density lipoprotein cholesterol (HDL-C) showed a significant increase when compared with HFHCgroup. On the other hand the results showed a significant increment in reduced glutathione level (GSH) in blood and liver. The percentage of changes in GSH in liver showed13.82 % and 21.47 % as well as, super oxide dismutase (SOD) activity by 33.05 % and 42.39 %. while a significant decrement in malondialdehyde (MDA) in serum and liver, the percentage of decrement in liver by 38.47% and 46.36% in groups fed on HFHC diet and treated with barley or malted barley respectively when compared with group fed on HFHC(p<0.05).It can be concluded that barley or malted barley can reduce lipidsprofile and oxidative stress but malted barley is more effective than barley.