Caloric sweetener sucrose is an essential component of modern diet. However sucrose diet has an adverse effect on body weight and is associated with medical complications. So, substituting sucrose with low calorie sweeteners either synthetic as aspartame or natural as stevia is an efficacious weight management strategy. In the present investigation, total numbers of 64 albino rats were divided into four groups: the first group served as control receiving distilled water. The second group represented the sucrose group receiving 10 % sucrose solution (weight by volume). The third group served as aspartame group receiving aspartame (75 mg/kg body weight). The fourth group represented stevia group and received stevia (40 mg/kg body weight). Rats were subjected to different experimental regimens for two durations, short duration (30 days) and long duration (90 days). Histological investigation of liver sections from aspartame treated rats revealed fatty degeneration, necrosis of hepatic cells, dilatation and congestion of the central blood vessels with distortion of normal lobular architecture with increase in collagenous fibers while stevia treatment illustrated preserved normal hepatic pattern. Histochemical staining of Best Carmine stain and bromophenol blue in liver tissue revealed significant decrease in glycogen content and protein content respectively in liver aspartame group followed by sucrose group. On the other hand, image analysis of liver sections stained with Sudan III demonstrated significant elevation in fat content in sections from aspartame treated group followed by sucrose group when compared to control group. Stevia administration revealed no histological or histochemical changes in the liver tissue sections compared to control group.