The number and diversity of reported mycoviruses have significantly increased with the utilization of next generation sequencing technologies. In the present study, an Egyptian isolate of a known mycovirus was identified in Penicillium chrysogenum isolated from Damietta governorate, Egypt. The genome of the identified mycovirus is divided into four double-stranded RNA (dsRNA) segments, three of which were completely sequenced and analyzed (dsRNA1; 3562 nts, dsRNA2; 3198 nts and dsRNA4; 2902 nts). The nucleotide sequence of the fourth segment (dsRNA3) was only partially determined. DsRNAs 1 and 2 encode RNA-dependent RNA polymerase (RdRp) and capsid protein (CP), respectively. The mycovirus of the current study was identified as an isolate of Penicillium chrysogenum virus (PcV) based on the high RdRp identity (99.37%) in BLASTX searches. The untranslated regions (UTRs) of the identified dsRNAs were conserved as reported for other chrysoviruses. Based on the phylogenetic analysis, PcV belongs to the genus Alphachrysovirus of Chrysoviridae.