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In vitro Formation of Synthetic Seed from Microshoots of Begonia x hiemalis Fotch

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Last updated: 03 Jan 2025

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Abstract

Artificial seed or synthetic seed formation of Begonia x hiemalis Fotch var. Schwabenland Red had been induced from leaf explants in vitro. The explants were cultured on (Murashige and Skoog (MS) medium supplemented with combinations of 1.0 mg/l Benzylamino purine (BAP) and 1.0 mg/l Naphthalene acetic acid (NAA). After 8 weeks, regenerated microshoots were excised from leaf cultures and microshoots successfully encapsulated in 3% sodium alginate solution together withMS salt solution and polymerized within 1.0M CaCl2.2H2O solution for 30 minutes. The beads produced were with firm coats, round beads and uniform size and in good shape for handling. The results demonstrate that the optimum germination and survival rate of encapsulated matrix was in MS medium containing 3% sodium alginate solution supplemented with 3% sucrose, 1.0 mg/l BAP in combination with 1.0 mg/l NAA. The viability of the encapsulated micro shoots after storage period at 4 C was also determined. High germination rate (100%) was achieved after 1-3 months storage whereas low germination rate (7-53%) was obtained after 4-6 months storage. The seeds were also successfully germinated in three different germination media including MS basal, garden soil and vermiculite.

Keywords

Begonia x hiemalis Fotch, encapsulated microshoots, synthetic seeds, tissue culture

Authors

First Name

Asmah

Last Name

Awal

MiddleName

-

Affiliation

1MARA University of Technology, Negeri Sembilan Branch, 72000 Kuala Pilah, Negeri Sembilan, Malaysia

Email

asmah@nsembilan.uitm.edu.my

City

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Orcid

-

First Name

Rosna

Last Name

Taha

MiddleName

-

Affiliation

Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia.

Email

rosna@um.edu.my

City

-

Orcid

-

First Name

Nor

Last Name

Hasbullah

MiddleName

-

Affiliation

Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia

Email

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Orcid

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Volume

2

Article Issue

2

Related Issue

3875

Issue Date

2007-11-01

Receive Date

2018-11-08

Publish Date

2007-11-01

Page Start

189

Page End

192

Print ISSN

1687-5052

Online ISSN

2090-2786

Link

https://cat.journals.ekb.eg/article_18615.html

Detail API

https://cat.journals.ekb.eg/service?article_code=18615

Order

13

Type

Original Article

Type Code

644

Publication Type

Journal

Publication Title

Catrina: The International Journal of Environmental Sciences

Publication Link

https://cat.journals.ekb.eg/

MainTitle

In vitro Formation of Synthetic Seed from Microshoots of Begonia x hiemalis Fotch

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Article

Created At

22 Jan 2023