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18611

Morphological, Anatomical and Tissue Culture Studies on Xylocarpus granatum

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Last updated: 22 Jan 2023

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Abstract

Xylocarpus species are important endangered mangrove species in Malaysia. One of the economic importance of the species, particularly Xylocarpus granatum is for wood carving. Hence, there is an urgent need to conserve the species. In the present study, some morphological, anatomical and tissue culture studies were carried out on Xylocarpus granatum. To date, there is no record on detailed morphological study of the species. Therefore, it is one of the aims of the study to investigate the morphological characteristics of the species. Anatomical studies on the leaf and primary stem were also carried out. Scanning electron microscope study revealed the presence of sunken, anomocytic-type stomata on the abaxial surface of the leaf. Results from anatomical studies showed the presence of thick cuticle on both abaxial and adaxial surfaces of the leaf. Sunken stomata and thick cuticle are adaptations of mangrove species to reduce transpiration. Tannin cells were also observed in the leaf lamina and primary stem and this needs further investigation. Regeneration of this species from tissue culture had been attempted, however, only callus formation was observed. Formation of callus from leaf segments, young stems and flower buds were observed after three weeks being cultured on MS medium supplemented with 2.5mg/l 2,4, Dichlorophenoxyacetic acid (2,4-D). MS medium supplemented with 10 mg/l 2,4-D and 10mg/l Naphthalene acetic acid (NAA) managed to produce 87.5% callus after 14 days in culture when leaf explants were used. It was observed that more calli were formed when the cultures were maintained in the dark. At pH 4.0, the cultures seemed to form faster and more calli were formed. The addition of 0.1g/l sodium chloride (NaCl) to MS medium produced 70% callus. The callus produced was analysed to see whether they are embryogenic or nonembryogenic. A few callus cells were found to be in the globular stage of embryogenesis but failed to develop further. Work is in progress to induce somatic embryogenesis in this species.

Keywords

Morphology, anatomy, Xylocarpus granatum, tissue culture, Callus, Hormones

Authors

First Name

Noorma

Last Name

Haron

MiddleName

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Affiliation

Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia

Email

noorma@um.edu.my

City

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Orcid

-

First Name

Rosna

Last Name

Taha

MiddleName

-

Affiliation

Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia

Email

rosna@um.edu.my

City

-

Orcid

-

Volume

2

Article Issue

2

Related Issue

3875

Issue Date

2007-11-01

Receive Date

2018-11-08

Publish Date

2007-11-01

Page Start

159

Page End

162

Print ISSN

1687-5052

Online ISSN

2090-2786

Link

https://cat.journals.ekb.eg/article_18611.html

Detail API

https://cat.journals.ekb.eg/service?article_code=18611

Order

9

Type

Original Article

Type Code

644

Publication Type

Journal

Publication Title

Catrina: The International Journal of Environmental Sciences

Publication Link

https://cat.journals.ekb.eg/

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Article

Created At

22 Jan 2023