In vitro cultures of Gerbera jamesonii Bolus ex. Hook f. were initiated from leaf explants. White friable callus was formed after 4 weeks at an average of 70% onMurashige and Skoog (MS) medium supplemented with 2, 4 dichlorophenoxyacetic acid (2,4-D). The identification of somatic embryos at early stages was done by treatment with 2% Acetocarmine and 0.5% Evan's Blue (double staining method). The embryogenic head cells stained bright red (acetocarmine) and suspensor cells stained blue in an embryogenic mass. In the mass of non-embryogenic callus, cells did not show any organization of heads and suspensors and will stain blue with Evan's Blue. White-cream friable embryogenic callus of Gerbera jamesonii was formed after 4 weeks when leaf explants were cultured on MS medium supplemented with 0.1 mg/l-2.0 mg/l 2, 4-D, 3.0% sucrose and solidified with 0.8% agar. The embryogenic callus was transferred into MS suspension culture medium supplemented with 1.0 mg/l 2, 4-D and 0.1 mg/l Naphthalene acetic acid (NAA) and was subcultured at 10 days interval for 1 month. Subsequent withdrawal of 2, 4-D from induction medium resulted in the induction and growth of somatic cells. Somatic embryos formed at globular phase were then sieved and transferred into maturation medium. Heart, torpedo and cotyledon phases of somatic embryo were identified.