Samples of bird excreta including domestic pigeons were collected from farmer houses and aviary shops in rural and urban areas of Ismailia. The collected samples were mycologically processed for the occurrence of the pathogenic yeast, Cryptococcus neoformans. It was isolated mainly from pigeon excreta and from only one sample of colored birds excreta collected from decorated colored bird's shops. Pigeon excreta positive for Cryptococcus neoformans were suspended in sterilized phosphate buffered saline then three different doses of the supernatant were interaperitoneally injected into groups of experimental albino mice to assess its dissemination and the various pathological effects resulting from systemic infection. Mainly, pulmonary and occasional meningeal lesions were evident in the infected mice.