The regenerated shoots of Pineapple (Ananas comosus Cv. Smooth Coyenne) during the establishment stage were cultured individually on MS medium supplemented with BAP (2.0 mg/L) during the proliferation stage and IBA (2.0 mg) during the rooting stage. Various sugars (glucose, fructose and sucrose) and concentrations (0, 10, 20 and 30 mg/L) were tested. Copper and Boron microelements concentrations were also tested. Data indicated that all sucrose treatments enhanced the proliferation and rooting, however Glucose and fructose improved shoot length. Increasing copper sulfate to (0.05 mg/L) produced the best proliferation. Meanwhile, Boric acid at the rate of 12.4 mg/L was the most suitable concentration for improving rooting percentage.