Shoot multiplication for Ruta graveolens L. was achieved when shoot tips excised from mature plants and cultured on Murashige and Skoog (MS) medium containing BA at 0.5, 1.0 and 2.0 mg l-1 combined with NAA at 0.1, 0.2 and 0.3 mg l-1. Highest rate of shoot proliferation was obtained after 8 weeks with the medium supplemented with BA at 0.5 mg l-1 and 0.3 mg l-1 NAA. Complete plants with strong, fibrous roots were obtained after transferring the separated shoots into rooting medium (1/2 MS or MS media with or without NAA). The survival rate of transplants during acclimatization reached 100% and the plants grew normally under the greenhouse conditions.