Micropropagation was used to propagate the wild economic important plant; Capparis cartilaginea which is difficult to propagate in normal conditions. For establishment and multiplication, MS medium containing 0.1 mg/L NAA + 3 mg/L BA was the most suitable medium for Capparis cartilaginea shoot tip explants. This medium gave the highest percentage of survival (100%), axillary shoot formation (76.74%), mean number (3.2) and length (0.564 cm) of axillary shoots per explant. The concentrations of 0.5 and 1 mg 2iP/L were suitable for the elongation of one (3.82 cm) and three shoots (3.114 cm) explants, respectively. The highest rooting percentage (22.22%) was obtained on half strength MS agar gelled medium supplemented with NAA at 2 mg/L under six days of dark incubation. A comparative study through the nucleic acids content and the protein banding pattern was carried out between the in vitro produced plantlets and the mother plant in autumn and spring seasons. The concentrations of RNA and DNA as well as the number of protein bands in the plantlets produced from tissue culture were increased over those of the newly growing parts of the mother plant.